Coding

Part:BBa_K5310060:Design

Designed by: Ilias Pilianidis   Group: iGEM24_thessaloniki   (2024-10-01)


MOG modular extracellular


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 14
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is designed as a modular head for the CAAR receptor we created in our project. This enables the extracellular part of the receptor to be inerchangeable. It has both BsmBI restriction sites and the restriction sites needed in order for us to be able to clone it in our PGK C1 acceptor plasmid after it is fused with the modular transmembrane-intracellular part of the CAAR.


Source

The sequence for this part comes from the fusion of the human MOG (aa 35-55) protein domain and the Igk signal peptide.

References